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Isolation of DNA from B cells and real-time PCR.PBMCs were isolated from whole blood and stored in liquid nitrogen. Forty-eight hours after transfection, cells were treated with 1 mM phenformin or vehicle for 24 hr before being imaged. Un grand nombre de ces effets secondaires peuvent être pris en charge et quelques-uns peuvent disparaître d'eux-mêmes avec le temps.
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The lentivirus generated is useful for transduction of broad range of cell types, including hard-to-transfect cell lines.
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